Protein Variants | Comment | Organism |
---|---|---|
H106C | the peroxidatic-to-catalatic ratio of the mutant is increased 125fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
H106Y | the peroxidatic-to-catalatic ratio of the mutant is increased 321fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
H267Y | the peroxidatic-to-catalatic ratio of the mutant is increased 36fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
R102C | the peroxidatic-to-catalatic ratio of the mutant is increased 18fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
R102K | the peroxidatic-to-catalatic ratio of the mutant is increased 7fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
R102L | the peroxidatic-to-catalatic ratio of the mutant is increased 13fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
W105F | the peroxidatic-to-catalatic ratio of the mutant is increased 2800fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
W105L | the peroxidatic-to-catalatic ratio of the mutant is increased 612fold, the heme content of the mutant is reduced relative to the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Escherichia coli | |
cyanide | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C | Escherichia coli | |
0.055 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, wild type enzyme, at pH 6.0 and 25°C | Escherichia coli | |
0.08 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C | Escherichia coli | |
0.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C | Escherichia coli | |
0.24 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C | Escherichia coli | |
0.33 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C | Escherichia coli | |
0.4 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C | Escherichia coli | |
0.48 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C | Escherichia coli | |
5.9 | - |
H2O2 | catalase activity, wild type enzyme, at pH 7.0 and 37°C | Escherichia coli | |
12 | - |
H2O2 | catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C | Escherichia coli | |
28 | - |
H2O2 | catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C | Escherichia coli | |
31 | - |
H2O2 | catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C | Escherichia coli | |
33 | - |
H2O2 | catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C | Escherichia coli | |
53 | - |
H2O2 | catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C | Escherichia coli | |
77 | - |
H2O2 | catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C | Escherichia coli | |
91 | - |
H2O2 | catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C | Escherichia coli | |
100 | - |
H2O2 | catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli UM262 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C | Escherichia coli |
0.25 | - |
catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C | Escherichia coli |
0.95 | - |
catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C | Escherichia coli |
1.9 | - |
catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C | Escherichia coli |
5.9 | - |
catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C | Escherichia coli |
10 | - |
catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C | Escherichia coli |
11 | - |
peroxidase activity, mutant enzyme H106C, at pH 7.0 and 37°C | Escherichia coli |
12 | - |
peroxidase activity, mutant enzyme H267Y, at pH 7.0 and 37°C | Escherichia coli |
18 | - |
peroxidase activity, mutant enzyme H106Y, at pH 7.0 and 37°C | Escherichia coli |
19 | - |
catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C | Escherichia coli |
35 | - |
catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C | Escherichia coli |
63 | - |
peroxidase activity, mutant enzyme R102C, at pH 7.0 and 37°C | Escherichia coli |
85 | - |
peroxidase activity, mutant enzyme R102K, at pH 7.0 and 37°C | Escherichia coli |
85 | - |
peroxidase activity, mutant enzyme R102L, at pH 7.0 and 37°C | Escherichia coli |
660 | - |
peroxidase activity, wild type enzyme, at pH 7.0 and 37°C | Escherichia coli |
1300 | - |
peroxidase activity, mutant enzyme W105L, at pH 7.0 and 37°C | Escherichia coli |
1900 | - |
catalase activity, wild type enzyme, at pH 7.0 and 37°C | Escherichia coli |
1900 | - |
peroxidase activity, mutant enzyme W105F, at pH 7.0 and 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Escherichia coli UM262 | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
H2O2 | - |
Escherichia coli | O2 + H2O | - |
? | |
H2O2 | - |
Escherichia coli UM262 | O2 + H2O | - |
? | |
isoniazid + H2O2 | - |
Escherichia coli | oxidized isoniazid + H2O | - |
? | |
isoniazid + H2O2 | - |
Escherichia coli UM262 | oxidized isoniazid + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Escherichia coli | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Escherichia coli UM262 | oxidized o-dianisidine + H2O | - |
? | |
tert-butyl hydroperoxide + H2O2 | - |
Escherichia coli | oxidized tert-butyl hydroperoxide + H2O | - |
? | |
tert-butyl hydroperoxide + H2O2 | - |
Escherichia coli UM262 | oxidized tert-butyl hydroperoxide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPI | - |
Escherichia coli |
hydroperoxidase I | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
H2O2 | catalase activity, mutant enzyme H106Y, at pH 7.0 and 37°C | Escherichia coli | |
0.089 | - |
H2O2 | catalase activity, mutant enzyme H106C, at pH 7.0 and 37°C | Escherichia coli | |
1.1 | - |
H2O2 | catalase activity, mutant enzyme W105F, at pH 7.0 and 37°C | Escherichia coli | |
1.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme H106Y, at pH 6.0 and 25°C | Escherichia coli | |
1.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme H106C, at pH 6.0 and 25°C | Escherichia coli | |
2.1 | - |
H2O2 | catalase activity, mutant enzyme R102K, at pH 7.0 and 37°C | Escherichia coli | |
2.6 | - |
H2O2 | catalase activity, mutant enzyme H267Y, at pH 7.0 and 37°C | Escherichia coli | |
23 | - |
H2O2 | catalase activity, mutant enzyme W105L, at pH 7.0 and 37°C | Escherichia coli | |
53.5 | - |
H2O2 | catalase activity, mutant enzyme R102C, at pH 7.0 and 37°C | Escherichia coli | |
660 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102L, at pH 6.0 and 25°C | Escherichia coli | |
710 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102C, at pH 6.0 and 25°C | Escherichia coli | |
900 | - |
H2O2 | catalase activity, mutant enzyme R102L, at pH 7.0 and 37°C | Escherichia coli | |
3700 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme R102K, at pH 6.0 and 25°C | Escherichia coli | |
5300 | - |
H2O2 | catalase activity, wild type enzyme, at pH 7.0 and 37°C | Escherichia coli | |
17000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, wild type enzyme, at pH 6.0 and 25°C | Escherichia coli | |
140000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme W105L, at pH 6.0 and 25°C | Escherichia coli | |
210000 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidase activity, mutant enzyme W105F, at pH 6.0 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.008 | - |
mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.008 | - |
wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.01 | - |
mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.012 | - |
mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.04 | - |
mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.055 | - |
mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.065 | - |
mutant enzyme W105L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.075 | - |
mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.09 | - |
wild type enzyme, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.095 | - |
mutant enzyme R102L, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.18 | - |
mutant enzyme R102C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.19 | - |
mutant enzyme H106C, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.21 | - |
mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide | |
0.23 | - |
mutant enzyme H106Y, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.25 | - |
mutant enzyme W105F, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | azide | |
0.39 | - |
mutant enzyme R102K, at pH 7.0 and 37°C for catalase activity and at pH 6.0 and 25°C for peroxidase activity | Escherichia coli | cyanide |